Effect of L-NMMA on the expression of GATA-2 and Epo mRNA.
(A) Northern blot analysis was performed using 20 μg of RNA from Hep3B cells incubated under conditions of normoxia (21% O2) (lane 1), conditions of hypoxia (1% O2) (lane 2), hypoxia with 10−5 mol/L L-NMMA (lane 3), hypoxia with 10−4 mol/L L-NMMA (lane 4), and hypoxia with 10−3 mol/L L-NMMA (lane 5) for 8 hours. Upper, middle, and lower panels show GATA-2, Epo, and 28S mRNA, respectively. (B) Quantitative differences in GATA-2 mRNA levels were evaluated by molecular imager FX. Abundance of GATA-2 mRNA is expressed as units of densitometry relative to normoxia. Three separate experiments were performed (n = 3). *Significance compared with normoxia,P < .01. **Significance compared with hypoxia,P < .05. ***Significance compared with hypoxia,P < .01.
