Canine vWF cDNA clones.

Clones obtained by screening a λgt11 canine heart cDNA library are shown schematically with reference to the full-length cDNA. The 5′ and 3′ ends of the inserts are indicated, with base 1 the adenine of the initiator methionine. Bold lines represent clones obtained in a first round of screening using mixed human and canine vWF probes. A second round of screening was performed using 2 new canine probes consisting of the 5′ end of clone 1.2 and the 3′ end of clone 19.1. Thin lines represent clones from the rescreening. Clone 5G3 was obtained by PCR amplification across the only remaining gap, between bases 3049 and 3287, and is shown as a dashed line. Clones 2B1, 1.2, 5G3, 19.1, and 6B1 were linked as a full-length cDNA in a series of ligations to produce the construct pKVneo. Restriction site junctions are depicted as vertical lines.