Multimeric structure of expressed human and canine vWF.

Canine vWF has a multimer structure similar to that of human vWF. The multimeric structure of expressed vWF constructs was analyzed nonreduced on a 2% agarose–SDS gel. All samples were run on the same gel, and intervening (nonrelevant) lanes have been removed for clarity. The mock-transfected control is shown in lane 1. Normal human plasma vWF (lane 2) shows a full range of multimers, whereas human Δpro expressed alone did not form multimers (lane 3). Expression intrans with human vW AgII resulted in full multimerization (lane 4), similar to that expressed for human wild-type vWF (lane 5). Expressed canine wild-type vWF forms multimers (lane 6) similar to canine Δpro expressed in trans with canine vW AgII (lane 7). Canine plasma vWF is shown in lane 8.