Fig. 2.
Fig. 2. Kinetic of CCR5, CXCR4, and HLA-DR expression and HIV expansion after antigenic stimulation. / (A) Positively selected Vβ3-, Vβ14-, and Vβ17-bearing CD4 T cells were stimulated with SEB (20 ng/mL) in the presence of autologous APCs and the surface expression of CCR5, CXCR4, and HLA-DR was analyzed by flow cytometry. Two representative results from 8 different blood donors are shown. (B) The SEB-reactive CD4+ T cells were stimulated and infected with HIV-1Lai and HIV-1BaL. HIV-p24 was determined in the cell-free culture supernatant by ELISA. (+/−) and (+/+) characterizes the genotype of CCR5 of the respective blood donors A and B as determined by PCR. (−) represents the 32-bp deletion of one allele. A comparable HIV expansion was observed with T cells from 4 different blood donors.

Kinetic of CCR5, CXCR4, and HLA-DR expression and HIV expansion after antigenic stimulation.

(A) Positively selected Vβ3-, Vβ14-, and Vβ17-bearing CD4 T cells were stimulated with SEB (20 ng/mL) in the presence of autologous APCs and the surface expression of CCR5, CXCR4, and HLA-DR was analyzed by flow cytometry. Two representative results from 8 different blood donors are shown. (B) The SEB-reactive CD4+ T cells were stimulated and infected with HIV-1Lai and HIV-1BaL. HIV-p24 was determined in the cell-free culture supernatant by ELISA. (+/−) and (+/+) characterizes the genotype of CCR5 of the respective blood donors A and B as determined by PCR. (−) represents the 32-bp deletion of one allele. A comparable HIV expansion was observed with T cells from 4 different blood donors.

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