Fig. 2.
![Fig. 2. Development of IL-3–dependent bone marrow–derived mast cells (BMMCs) is normal in γc− and Jak3− mice. / (A) BMMCs (2 × 105/well) were cultured in the presence of IL-3 at 37°C for 36 hours, and the proliferative responses were evaluated by the addition of [3H] thymidine for the final 12 hours. Data are means ± SD for 5 mice in each group. (B) BMMCs from WT, γc−, and Jak3− mice were stained with anti–c-kit APC, anti-FcγII/III PE, and IgE FITC, as described in “Materials and methods.” Shown are representative FACS profiles for c-kit, FcγII/III, and IgE receptor staining from 5 independent experiments. Dashed lines are FACS profiles of negative controls.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/6/10.1182_blood.v96.6.2172/5/h81800149002.jpeg?Expires=1735511551&Signature=PwyncQjSwPhui3CGGcYD2DNxp7qcxeqTUfPjgrNE0JOuizMf~amlu2Xz1~MAW-XUPYkzvP91lhJmwJ4Se69xOL7cknXVE2Uz-uRUg1lJXtjdoJEAcfgyfy4fYVAHM6wT4ZgpPeGQQ5YcSbyhv9ya-jlRx34dU0EGmESf6-O6Hq89IjMXPqw4j3vdvHK0eE3Rw9CcZilgzF5GM2WkXxEm3m45UZPZwn675evJ8wk4-N-8RYOM1OnzJY18nc9Kh9yOHwpDVq0YZbl5~Z-K5SHeMKlRNZHU1-Y9Q0pePJNBWX~Soo7i3G1~fGthP4bg6eKIROtWTsVeRbL4deTZF1vX2g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Development of IL-3–dependent bone marrow–derived mast cells (BMMCs) is normal in γc− and Jak3− mice.
(A) BMMCs (2 × 105/well) were cultured in the presence of IL-3 at 37°C for 36 hours, and the proliferative responses were evaluated by the addition of [3H] thymidine for the final 12 hours. Data are means ± SD for 5 mice in each group. (B) BMMCs from WT, γc−, and Jak3− mice were stained with anti–c-kit APC, anti-FcγII/III PE, and IgE FITC, as described in “Materials and methods.” Shown are representative FACS profiles for c-kit, FcγII/III, and IgE receptor staining from 5 independent experiments. Dashed lines are FACS profiles of negative controls.
