IL-4–induced phosphorylation of 65-kd Stat6 isoform in BMMCs is γ_c- and Jak3-dependent.

BMMCs from WT, γ_c⁻, and Jak3⁻mice were washed with PBS, cultured for 2 hours in the absence of IL-3, and stimulated with IL-4 or IL-13 (20 ng/mL) for 15 minutes. After washing with PBS, cell lysates were prepared, separated on a 10% SDS gel, and blotted with antisera to either phospho-Stat6 or Stat6 (M200). As a control, cell lysates from IL-4–stimulated WT splenocytes were used. Shown is a representative blot from 4 independent experiments.