Surface phenotype of human blood monocyte–derived DCs stimulated by LPS (MADCs).

Human monocytes were cultured in RPMI 1640 medium plus 7.5% FCS in the presence of GM-CSF (500 U/mL) and IL-4 (100 U/mL) for 5 days and then stimulated with LPS for 2 days. After culture, the cells were washed and then stained with various antibodies as described in “Materials and methods.” The data are shown as histograms depicting the number of cells exhibiting various fluorescence intensities. The dotted lines represent MADCs stained with specific antibodies, the solid histograms represent IMDCs stained with specific antibodies, and the solid lines represent isotype-matched control. Results are representative of 3 independent experiments.