Western blot analysis of RARα in selected RARα-transfected D⁺ cell clones after 24-hours incubation with 3 μmol/L ATRA.

Loading was standardized by equal cell numbers. Coomassie staining of a duplicate gel shows approximately equal amounts of protein in each lane. D⁺V indicates vector-transfected D⁺ cells; C, untreated control; R, ATRA (3 μmol/L).