Morphological changes characteristic of apoptosis, and time-dependent effects of BMP-2 on cellular proliferation.

U266 cells and HS-Sultan cells were treated with BMP-2 (50 ng/mL) for 48 hours and 72 hours, respectively. Cytospin slides were prepared and stained with Giemsa. Original magnification, ×1000. Cell viability was measured by MTT assay as compared with control cells cultured without BMP-2.