American Society of Hematology

Fig. 2.

$Fig. 2. Autologous DCs pulsed with EBV B-LCL–derived freeze–thaw lysate induces EBV-specific CD4+T-cell responses in an antigen dose-dependent manner. / CD4+ T cells directly isolated from blood lymphocytes of healthy anti-EBV–positive donor IP1 were seeded at 105cells per well and were tested for reactivity against freeze–thaw lysate fractions 10 kd or larger prepared from autologous LCL cells, B-, or T-cell blasts in IFN-γ Elispot assays. For antigen processing and presentation, autologous immature DCs were prepulsed with freeze–thaw cell lysates at the ratio of cell equivalents per DCs of 100:1, 10:1, or 1:1. Resulting spots were evaluated and presented as described in Figure 1. Results were confirmed in 3 independent experiments.$

Autologous DCs pulsed with EBV B-LCL–derived freeze–thaw lysate induces EBV-specific CD4⁺T-cell responses in an antigen dose-dependent manner.

CD4⁺ T cells directly isolated from blood lymphocytes of healthy anti-EBV–positive donor IP1 were seeded at 10⁵cells per well and were tested for reactivity against freeze–thaw lysate fractions 10 kd or larger prepared from autologous LCL cells, B-, or T-cell blasts in IFN-γ Elispot assays. For antigen processing and presentation, autologous immature DCs were prepulsed with freeze–thaw cell lysates at the ratio of cell equivalents per DCs of 100:1, 10:1, or 1:1. Resulting spots were evaluated and presented as described in Figure 1. Results were confirmed in 3 independent experiments.

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