Accelerated tumorigenesis in
Notch1ΔC/E2A-PBX1double transgenic mice. (A) Schematic illustration of the transgene construct consisting of aNotch1ΔC cDNA under control of theLCK proximal promoter. (B) The survival for cohorts ofNotch1ΔC/E2A-PBX1 (n = 10) orNotch1/E2A-PBX1 (n = 12) double transgenic mice is compared with that for littermates that are singly transgenic for either E2A-PBX1 (n = 10), Notch1 (n = 10), orNotch1ΔC (n = 10). Deaths were the result of malignant lymphoma confirmed by histologic examination. (C) Southern blot analysis of T-cell receptor gene rearrangements in lymphomas. Dash indicates migration of germline bands. (D) Quantitative RT-PCR analysis of transgene RNA expressed in thymi from wild-type and transgenic mice identified at the top of the gel lanes. Products derived from β-actin control and Notch transgenes are indicated to the right. (E) FACS analysis of CD4 and CD8 expression in thymocytes of 12-week-old wild-type, Notch1ΔC or E2A-PBX1single transgenic and Notch1ΔC/E2A-PBX1 double transgenic mice.
