American Society of Hematology

Fig. 1.

$Fig. 1. CD4 and F4/80 expression by splenic DCs. / (A) Phenotype of CD8α+ and CD8α− DCs analyzed on splenic DC-enriched very low density fractions obtained without antibody-mediated depletion. Black histograms show the proportion of CD8α− and CD8α+ DCs after gating for CD11c+ cells and the proportion of CD4− and CD4+ DCs within the CD8α− subset. White profiles represent control stainings. The percentage of cells positive for the indicated markers is shown under the gray histograms. (B) Splenic CD11c versus CD8α profiles of control mice and mice injected intravenously with in vivo depleting anti-CD4 antibodies, showing that most CD8α−DCs were eliminated after anti-CD4 treatment. Data are representative of 3 experiments with similar results.$

CD4 and F4/80 expression by splenic DCs.

(A) Phenotype of CD8α⁺ and CD8α⁻ DCs analyzed on splenic DC-enriched very low density fractions obtained without antibody-mediated depletion. Black histograms show the proportion of CD8α⁻ and CD8α⁺ DCs after gating for CD11c⁺ cells and the proportion of CD4⁻ and CD4⁺ DCs within the CD8α⁻ subset. White profiles represent control stainings. The percentage of cells positive for the indicated markers is shown under the gray histograms. (B) Splenic CD11c versus CD8α profiles of control mice and mice injected intravenously with in vivo depleting anti-CD4 antibodies, showing that most CD8α⁻DCs were eliminated after anti-CD4 treatment. Data are representative of 3 experiments with similar results.

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