T-cell stimulation capacity of CD8α⁺ DCs versus CD8α⁻ DCs.

T-cell stimulatory capacity of CD8α⁺ versus CD8α⁻ DC (A) and CD4⁻ CD8α⁻versus CD4⁺ CD8α⁻ DC (B). DCs from C57BL/6 mice were cultured with purified allogeneic T cells from BALB/c mice at the indicated APC:T cell ratios. After 5 days, T-cell proliferation was determined by [³H] thymidine uptake or CD25 expression. Data are representative of 3 experiments with similar results. In the [³H] thymidine incorporation assay, error bars represent the SD for triplicate cultures.