Reconstitution of splenic CD8α⁺ and CD8α⁻ DCs from CD44⁺ CD25⁺precursors.

Thymic CD44⁺ CD25⁺ precursors (3 × 10⁴) were injected intravenously into 7 Gy γ-irradiated 8-week-old C57 BL/6 Ly 5.1 Pep^3b recipient mice, along with 4 × 10⁴ Ly 5.1 BM cells to ensure survival of recipients. Ten days after transfer of precursors, mice were analyzed for donor-derived DCs. The percentage of Ly5.2⁺ CD11c⁺ DCs as well as the percentage of CD8α⁻ and CD8α⁺ DCs among Ly5.2⁺ DCs are indicated. Data are representative of 2 independent experiments with similar results.