Fig. 5.
Stable and ectopic expression of the MgcRacGAP transgene in HL-60 and M1 transfectants.
(A) Northern blot analysis of mRNA from parental HL-60 cells, HL-60/pMX-IRES-EGFP cells, and HL-60/pMX-MgcRacGAP-IRES-EGFP cells (lanes 1, 2, and 3, respectively). (B) Western blot analysis of cell lysates from control M1 cells (lanes 1,3) and M1 cells with the introduced MgcRacGAP transgene (lanes 2,4) (3 × 107per lane).