GPIIb-IIIa incorporation into the cytoskeleton.

Using the samples from the gel in Figure 3, Western blots were performed and probed using a polyclonal antibody against GPIIb-IIIa. The gel shown is representative of 3 experiments. Lane 1 contains spontaneous aggregation; lane 2, D3 aggregated platelets; lane 3, ADP aggregated platelets; lane 4, D3/ADP added simultaneously; lane 5, D3 2 minutes of preincubation and ADP; lane 6, D3 and ADP preincubation for 2 minutes; lane 7, whole platelets; lane 8, molecular weight standards.