Bcr/Abl and STAT5-1*6 increase Bcl-XLprotein expression.
(A) Ton.B.210 cells were cultured in RPMI 1640 + 10% FCS. At Time 0, 1 μg/mL doxycycline was added to the cell culture and part of the cells were harvested at the indicated time points. Cells were lysed, and an equal amount of protein was separated by SDS-PAGE, and transferred to PVDF membrane. The membrane was cut in strips and probed with the indicated antibodies. The p85-PI3K blot is used as a control for equal loading. (B) Ton.B.1*6 cells were induced with 1 μg/mL doxycycline in RPMI 1640 10% FCS alone or supplemented with IL-3. Part of the cells were harvested at the indicated time points. Cells were lysed, and an equal amount of protein was separated by SDS-PAGE and transferred to PVDF membrane. The membrane was cut in strips and probed wit the indicated antibodies.
