Activity of the CCR3 promoter in noneosinophilic cell lines.

Cells were transiently transfected using the Effectene method with a reporter plasmid containing 1.6 kb of human CCR3 promoter or no promoter ligated to the luciferase gene. Cells were cotransfected with the pcDNA3.βGal plasmid and the data are normalized to β-galactosidase activity. Cell lines shown are A, U937; B, L1.2; C, Jurkat; and D, A549. A dose response of CCR3 promoter activity is depicted as well as the value of the promoterless vector (basic). The control vector was used at 1 μg in A through C and 0.4 μg in D. On the y-axis, data are presented as RLU (luciferase activity)/OD (β-galactosidase activity). Data are presented for a representative experiment (n = 2-4 for each cell line).