Activity of CCR3 promoter deletion constructs.

A schematic representation of the deletion constructs cloned into the pGL3 luciferase vector is shown in the left panel. The promoter sequence is shown as a line and exon 1 is depicted as an open box. The position of the KpnI restriction site and the deletion construct end positions are shown with arrowheads. In the right panel, cells were transfected with the reporter plasmid indicated. In A and B, A549 cells were cotransfected with the pcDNA3.βGal plasmid and the data are normalized to β-galactosidase activity. On the x-axis, data are presented as RLU (luciferase activity)/OD (β-galactosidase activity). In C, AML14.3D10 cells were cotransfected with the pRL.SV40 and the data are normalized to the activity of the renilla luciferase. On the x-axis, data are presented as RLU1 (firefly luciferase activity)/RLU2 (renilla luciferase activity). Data are presented for a representative experiment (n = 3). Each point was performed in triplicate and is expressed as mean ± SD.