Induced expression of CD80/B7-1 after huCD40LT stimulation of primary MCL cell cultures.
Isolated mononuclear cells from 6 MCL patients were thawed at day 0 and analyzed using flow cytometry, or seeded at 2 × 106cells/mL and cultured in the presence or absence of 400 ng/mL for 4 days. Before and after culture, the harvested cells were incubated with anti-CD19 (FITC) and anti-CD80/B7-1 (PE) antibodies. A total of 5000 cells were counted using standard flow cytometry analysis. The 2 MCL patients shown represent one case with low (no. 5) and one with high (no. 3) proliferative response to huCD40LT stimulation, respectively. Dot-plot analyses are shown from day 0 and after 4 days of culture, in the absence (day 4: control) or presence of huCD40LT (day 4: huCD40LT) from the 2 MCL patients. sCD40L indicates huCD40LT stimulation.
