American Society of Hematology

Fig. 1.

$Fig. 1. Pretreatment of HUVEC with mononuclear leukocytes from patients with sickle cell disease increases the adhesion of normal PMNL to HUVEC relative to media alone or to mononuclear leukocytes from normal subjects. / HUVEC were pretreated with media alone, TNF-α (10 ng/mL), or sickle or normal mononuclear leukocytes (2 leukocytes/endothelial cell) for 4 hours at 37°C. After the 4-hour pretreatment period, the endothelial monolayer was washed 3 times, and 51Cr-labeled normal PMNL were added to HUVEC and incubated for 15 minutes. HUVEC were washed 3 times, and PMNL adhesion to endothelium was calculated by counting radioactivity in the cell bound and unbound wash fractions. Results are expressed as percentage of PMNL bound to HUVEC. *P < .05 for sickle versus normal mononuclear leukocytes.$

Pretreatment of HUVEC with mononuclear leukocytes from patients with sickle cell disease increases the adhesion of normal PMNL to HUVEC relative to media alone or to mononuclear leukocytes from normal subjects.

HUVEC were pretreated with media alone, TNF-α (10 ng/mL), or sickle or normal mononuclear leukocytes (2 leukocytes/endothelial cell) for 4 hours at 37°C. After the 4-hour pretreatment period, the endothelial monolayer was washed 3 times, and ⁵¹Cr-labeled normal PMNL were added to HUVEC and incubated for 15 minutes. HUVEC were washed 3 times, and PMNL adhesion to endothelium was calculated by counting radioactivity in the cell bound and unbound wash fractions. Results are expressed as percentage of PMNL bound to HUVEC. *P < .05 for sickle versus normal mononuclear leukocytes.

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