Sickle mononuclear leukocytes activate HUVEC expression of E-selectin, ICAM-1, VCAM-1, and tissue factor relative to normal mononuclear leukocytes.

HUVEC were incubated with media alone or sickle or normal mononuclear leukocytes (2 leukocytes/endothelial cell) for 5 hours at 37°C. HUVEC adhesion molecule and tissue factor proteins were measured by enzyme immunoassay, and the results are expressed as a percentage of control (media only) HUVEC (100%). *P < .05 for sickle versus normal mononuclear leukocytes.