Fig. 5.
Adhesion of S. aureus to vWF surfaces.
PMMA coverslips were preadsorbed with indicated concentrations of vWF, then incubated with [3H]-thymidine–labeled cells (4 × 106 CFU/mL) of either S. aureus 8325-4 (A), Cowan 1 (B), the respective Δspa mutants, or the pSPA7235-complemented Δspa mutant in PBS/HSA (60 minutes at 37°C). ▪, wild-type strains; ●, Δspa mutants; ♦, complemented mutant. Shown are mean values ± SD of 3 determinations performed in quintuplicate.