American Society of Hematology

Fig. 6.

$Fig. 6. Thrombopoietin, but not IFN-α, induces SOCS-3 protein expression. / Cell lysates from BaF3/mMpl were prepared from unstimulated cells or those grown for 4 hours with 10 ng/mL TPO, 500 U/mL IFN-α, or both. Thirty minutes before lysate preparation, a proteosome inhibitor, MG132, was added to the cells. A polyvalent antiserum raised against the carboxyl terminus of SOCS-3 was used to immunoprecipitate specific protein, which was then size fractionated by SDS-PAGE, transferred to nitrocellulose, and probed for SOCS-3 with a monoclonal antibody to the protein. This experiment was repeated 3 times with identical results. Relative molecular weight markers are shown on the left in kilodaltons.$

Thrombopoietin, but not IFN-α, induces SOCS-3 protein expression.

Cell lysates from BaF3/mMpl were prepared from unstimulated cells or those grown for 4 hours with 10 ng/mL TPO, 500 U/mL IFN-α, or both. Thirty minutes before lysate preparation, a proteosome inhibitor, MG132, was added to the cells. A polyvalent antiserum raised against the carboxyl terminus of SOCS-3 was used to immunoprecipitate specific protein, which was then size fractionated by SDS-PAGE, transferred to nitrocellulose, and probed for SOCS-3 with a monoclonal antibody to the protein. This experiment was repeated 3 times with identical results. Relative molecular weight markers are shown on the left in kilodaltons.

This Feature Is Available To Subscribers Only