LTC-IC activity of CD34⁺CD38⁺and CD34⁺CD38⁻ cells in 5q− MDS patients.

Five-week LTC-IC activity of MDS BM CD34⁺CD38⁺cells (A) and CD34⁺CD38⁻ cells (B) was evaluated as described in “Materials and methods.” CD34⁺CD38⁺ and CD34⁺CD38⁻ cells isolated from normal subjects were included as controls. After 5 weeks of culture, the number of CFCs produced was evaluated in methylcellulose, as described in “Materials and methods.” Results are the means of 3 to 6 replicate wells from each cell population and patient.