Fig. 2.
Fig. 2. Effect of overexpressed SDF-1 or intrakine on the expression and function of CXCR4 in reconstituted mice. / (A) Cell surface expression of CXCR4 on the splenocytes (i, iii, v) and B220+ cells (ii, iv, vi) of peripheral blood from reconstituted mice. Cells from each reconstituted mouse at 70 days after transplantation were stained with control antibody (dotted lines), anti-mCXCR4 (bold lines), or without antibody (solid lines) and were subjected to flow cytometric analysis. Panels i and ii show cells from GFP-transduced mice. Panels iii and iv show cells from SDF-1–transduced mice. Panels v and vi show cells from intrakine-transduced mice. (B, C) Cell surface expression of CCR7 and CCR1 on the splenocytes from reconstituted mice. Cells were stained with control antibody (dotted lines), anti-chemokine receptor (bold lines), or without antibody (solid lines). These results represent 4 independent experiments.

Effect of overexpressed SDF-1 or intrakine on the expression and function of CXCR4 in reconstituted mice.

(A) Cell surface expression of CXCR4 on the splenocytes (i, iii, v) and B220+ cells (ii, iv, vi) of peripheral blood from reconstituted mice. Cells from each reconstituted mouse at 70 days after transplantation were stained with control antibody (dotted lines), anti-mCXCR4 (bold lines), or without antibody (solid lines) and were subjected to flow cytometric analysis. Panels i and ii show cells from GFP-transduced mice. Panels iii and iv show cells from SDF-1–transduced mice. Panels v and vi show cells from intrakine-transduced mice. (B, C) Cell surface expression of CCR7 and CCR1 on the splenocytes from reconstituted mice. Cells were stained with control antibody (dotted lines), anti-chemokine receptor (bold lines), or without antibody (solid lines). These results represent 4 independent experiments.

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