Fig. 3.
Fig. 3. Chemotactic analysis. / (A) Splenocytes from GFP-transduced (open column), SDF-1–transduced (hatched column), or intrakine-transduced (gray column) mice were stimulated with the indicated concentration of SDF-1, SLC, or MIP-1α in a 96-well chemotaxis chamber. The assay was performed in triplicate, and the number of migrated cells was counted by a flow cytometer. Each point represents mean ± SE from 4 separate experiments. Statistical analysis was performed by Student t test (*P < .05; **P < .01). (B) Chemotactic analysis on bone marrow cells. Bone marrow cells from GFP-transduced (open column), SDF-1–transduced (hatched column), or intrakine-transduced (gray column) mice were stimulated with the indicated concentration of SDF-1 in a 96-well chemotaxis chamber. The assay was performed in triplicate, and the number of migrated cells was counted by a flow cytometer. Each point represents the mean ± SE from 4 separate experiments. *P < .01.

Chemotactic analysis.

(A) Splenocytes from GFP-transduced (open column), SDF-1–transduced (hatched column), or intrakine-transduced (gray column) mice were stimulated with the indicated concentration of SDF-1, SLC, or MIP-1α in a 96-well chemotaxis chamber. The assay was performed in triplicate, and the number of migrated cells was counted by a flow cytometer. Each point represents mean ± SE from 4 separate experiments. Statistical analysis was performed by Student t test (*P < .05; **P < .01). (B) Chemotactic analysis on bone marrow cells. Bone marrow cells from GFP-transduced (open column), SDF-1–transduced (hatched column), or intrakine-transduced (gray column) mice were stimulated with the indicated concentration of SDF-1 in a 96-well chemotaxis chamber. The assay was performed in triplicate, and the number of migrated cells was counted by a flow cytometer. Each point represents the mean ± SE from 4 separate experiments. *P < .01.

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