Akt kinase activity in untreated and CGP57148B-treated cells.

Following treatment of HL-60/neo, HL-60/Bcr-Abl, and K562 cells with 0.5 μmol/L CGP57148B (CGP) for 48 hours, cell lysates were used to determine either the Akt kinase activity by measuring phosphorylation of its substrate GSK-3α, or a Western analysis of XIAP, cIAP1, and β-actin was performed (see text). Beta-actin served as the control for protein loading. CGP lowered Akt kinase activity as well as XIAP and cIAP1 levels in HL-60/Bcr-Abl and K562 but not HL-60/neo cells.