Loss of surface-expressed antigen induced by anti-GPIbα and anti-GPIIbIIIa mAbs.

(A) Two-color flow cytometric analysis of platelets from mice 6 hours after antibody injection. The data shown are representative of 6 mice per group. (B) Surface expression of GPIIbIIIa and P-selectin on platelets from mice 6 hours after injection of anti-GPIIbIIIa mAb (JON platelets). Resting (black bars) or thrombin-activated (gray bars) platelets were incubated with the indicated FITC-labeled antibody for 10 minutes at RT and analyzed directly.