Effects of repeated injections.

Mice received 7 injections of 7.5 μg mAb each intraperitoneally. (A) Platelet counts were determined at the indicated times, using an improved Neubauer hemocytometer. Results are shown as mean ± SD for groups of each 6 mice (○: IgG1; ■: IgG2a; ▿: IgG2b). (B) Hematocrits were determined at the indicated times. (C) Subcutaneous bleeding 48 hours after antibody injections.