Augmentation of interleukin (IL) 8 production in eosinophils primed with granulocyte-macrophage colony-stimulating factor (GM-CSF).

Cells were preincubated in 10 ng/mL GM-CSF. After 30 minutes, aliquots were distributed in wells containing medium, 50 ng/mL tumor necrosis factor α (TNF-α), 50 ng/mL IL-5, 0.7 μmol/L human recombinant histamine-releasing factor (HrHRF), or a combination of these cytokines, as indicated on the x-axis. After 24 hours of culture, supernatants were collected and IL-8 content was determined by enzyme-linked immunosorbent assay (ELISA). *P < .05 compared with cells incubated with medium alone (4 experiments).