American Society of Hematology

Fig. 2.

$Fig. 2. Characterization of the UCB SP for properties of HSCs. / (A,B) The expression of CD34 and CD38 on unfractionated UCB (A) and the UCB SP (B). The SP represented the dimmest 0.05% to 0.1% of the Hoechst-stained UCB. Quadrant statistics are provided for the individual experiment depicted. These data are representative of 5 experiments. (C) Hematopoietic progenitors from unfractionated UCB, CD34+ UCB, CD34+ UCB SP cells, and CD34− UCB SP cells were enumerated in LTCs on allogeneic bone marrow stroma. The data represent the mean ± SD of 3 experiments. Cultures of CD34+ UCB SP cells represent only 2 UCB samples; LTCs detected from CD34− SP cells were derived from 1 well of 8 wells plated.$

Characterization of the UCB SP for properties of HSCs.

(A,B) The expression of CD34 and CD38 on unfractionated UCB (A) and the UCB SP (B). The SP represented the dimmest 0.05% to 0.1% of the Hoechst-stained UCB. Quadrant statistics are provided for the individual experiment depicted. These data are representative of 5 experiments. (C) Hematopoietic progenitors from unfractionated UCB, CD34⁺ UCB, CD34⁺ UCB SP cells, and CD34⁻ UCB SP cells were enumerated in LTCs on allogeneic bone marrow stroma. The data represent the mean ± SD of 3 experiments. Cultures of CD34⁺ UCB SP cells represent only 2 UCB samples; LTCs detected from CD34⁻ SP cells were derived from 1 well of 8 wells plated.

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