Flow cytometric analysis of P-selectin expression.

(A) Analysis of P-selectin expression on resting HDMECs. (B) Analysis of P-selectin expression on HDMECs stimulated with 10 ng/mL of OSM for 48 hours. The thick line represents cells stained with a PE-labeled anti–P-selectin MoAb. The thin line represents cells stained with PE-labeled IgG1 control. The shift in mean fluorescent intensity for the unstimulated cells was from 7.38 to 9.29, and the shift for the OSM-stimulated cells was from 12.4 to 23.6. Results are representative of 3 HDMEC preparations. FL2-H indicates fluorescent intensity (phycoerythrin).