RT-PCR analysis of genes expressed differently in resting and LPS-stimulated monocytes.

(A) RT-PCR was performed on total RNA isolated from human resting monocytes (1), monocytes only incubated for 3 hours without LPS (2), and monocytes cultured for 3 hours with LPS (3), as described in Materials and methods. A, B, and C indicate different donors. Mono and LPS indicate resting monocytes and LPS-stimulated monocytes, respectively. SAGE tag No. is the number of times the tag was identified as shown in Tables 3 and 4. (B) Monocytes were incubated with LPS for 0, 1, 3, 6, 12, 24 hours, respectively, and RT-PCR was performed on these samples from 3 different donor-derived cells. A representative result is shown.