Detection of IL-9R–α transcripts in human peripheral blood eosinophils.

Preparation of human peripheral blood eosinophils from nonasthmatics (lanes 3 and 4), asthmatics (lanes 5 and 6), and eosinophil-differentiated HL-60 cell lines (lane 2) showed IL-9R–α–specific amplified fragments. The specificity of the amplified fragments was confirmed by Southern blot analysis using an internal primer. The control was β-actin, and cDNA was not used in lane 1. Eosinophil RNA was isolated, and first-strand cDNA synthesis was performed. Human IL-9R was amplified using PCR and IL-9R–α–specific primers on either side of a splice junction to preclude amplification of possible contaminating genomic DNA.