Targeting of cell surface molecules to the uropods of myeloma cell lines.

CAG cells (A-C and G-H), ARP-1 cells (F and I), U-266 cells (D-E and J-K), and ARK cells (L) were stained against CD44 (A), ICAM-1 (C-F), or syndecan-1 (G-L). ICAM-1 and syndecan-1 staining was performed after fixation of the cells, whereas CD44 staining was performed on live cells at 37°C. A-B, D-E, G-H, and J-K are corresponding visual fields. The figure shows polarization of these molecules to uropods in cells expressing uropods. Nonpolarized cells have uniform expression of these molecules over the entire cell surface, as shown for syndecan-1 in ARK cells (L).