CCR6 is not expressed during in vitro plasmablast differentiation of GC B cells.

(A) Phenotypic changes during the differentiation of GC B cells into plasmablasts in vitro. GC B cells purified by immunomagnetic cell sorting (> 90% cell viability) were cultured in the presence of IL-10, IL-2, IL-4, and anti-CD40 MoAb, as described in “Materials and methods.” At the indicated time points, CD38 and CD20 expression was determined by double staining with anti-CD38-PE and anti-CD20-FITC MoAbs. Note the progressive increase in the CD38^highCD20⁻ population corresponding to the plasmacytoid differentiation stage. Percentages of cells within each quadrant are indicated. (B) Analysis of CCR6 expression by double staining with anti-CD20-FITC and anti-CCR6-PE MoAbs during plasmablast differentiation is presented in panel A. One representative experiments of 3 using cells from different donors is shown.