Caspase involvement in As-IFN induced cell growth inhibition and apoptosis.

(A) Inhibition of cell growth. Effects of caspase inhibitor zVAD (50 μmol/L) on the growth inhibition induced by As-IFN in HuT-102 cells. Thymidine incorporation is expressed as percentage ± SD of control and represents the mean of 3 independent experiments. Actinomycin D– (100 ng/mL) treated MOLT-4 cells serve as control of caspase-dependant apoptosis. (B) TUNEL analysis of MOLT-4 and HuT-102 cell lines. Histogram analysis was achieved by setting a region for control cells and defining the region for positive TUNEL to include 3% of the normal cells.