Fig. 1.
Inducible expression of H-rasG12V in K562/ras, F-36P/ras, and 32D/ras.
(A) Western blot analysis on Ras expression during IPTG treatment. Each clone was treated with 0.5 mmol/L IPTG for the time indicated. Total cell lysates were subjected to SDS-PAGE and probed with anti-Ras antibody. (B) Light micrograph of K562/ras, F-36P/ras, and 32D/ras before and after 5-day culture with IPTG. Cytocentrifugation preparation from each culture was stained with May–Grünwald–Giemsa (original magnification, ×100).
