Fig. 3.
Fig. 3. GATA-1–transfected 32D/ras undergoes megakaryocytic differentiation in response to H-rasG12V. / (A) Light micrograph of 32D/ras/Mock and 32D/ras/GATA-1 before and after 5-day culture with IPTG. Cells were cultured with or without IPTG for 5 days and subjected to analysis. Cytocentrifugation preparation was stained with May–Grünwald–Giemsa (original magnification, ×100). (B) Flow cytometric analyses on DNA content and surface expression of CD61. DNA content of cultured cells was quantitated by propidium iodide staining and analyzed on FACSort. Surface expression of CD61 was analyzed by staining with anti-CD61 monoclonal antibody () and control antibody of the same isotype (····). (C) Changes in expression of GPIIb and PF4 mRNA in 32D/ras/GATA-1 during IPTG treatment.

GATA-1–transfected 32D/ras undergoes megakaryocytic differentiation in response to H-rasG12V.

(A) Light micrograph of 32D/ras/Mock and 32D/ras/GATA-1 before and after 5-day culture with IPTG. Cells were cultured with or without IPTG for 5 days and subjected to analysis. Cytocentrifugation preparation was stained with May–Grünwald–Giemsa (original magnification, ×100). (B) Flow cytometric analyses on DNA content and surface expression of CD61. DNA content of cultured cells was quantitated by propidium iodide staining and analyzed on FACSort. Surface expression of CD61 was analyzed by staining with anti-CD61 monoclonal antibody () and control antibody of the same isotype (····). (C) Changes in expression of GPIIb and PF4 mRNA in 32D/ras/GATA-1 during IPTG treatment.

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