Death receptor expression during maturation of human monocyte– derived dendritic cells.

(A) Immature DCs cultured for 7 days (d7) or 10 days (d10 immature) as well as mature DCs treated with IL-1α (500 IU/mL), IL6, TNF-α, IL-1β (all at 1000 IU/mL), and PGE₂ (10⁻⁸mol/L) from day 7 to day 10 (d10) were incubated with 5 μg/mL of monoclonal Ab to CD95 (DX2) or CD83, followed by FITC-conjugated secondary Ab and analyzed by FACS. (B) Cells were treated as described above and analyzed for the presence of TRAIL receptors (TRAIL-R) 1 to 4.