Effect of PU.1 on GATA-1 mRNA and protein expression in K562/PU.1 cells.

PU.1 does not inhibit GATA-1 mRNA and protein expression in K562/PU.1 cells. (A) Northern blot analysis of GATA-1 mRNA in K562/mPU.1 cells at different time points after induction of PU.1 expression with ZnSO₄. The ethidium bromide–stained 18S RNA was used as a loading control as shown at the bottom of the panel. (B) Western blot analysis of PU.1 and GATA-1 protein levels at different time points after ZnSO₄ induction in whole-cell lysates from K562/mPU.1 cells. β-Actin was used as a loading control. (C) Western blot analysis of PU.1 and GATA-1 in nuclear extracts from K562/mPU.1 cells after ZnSO₄ induction. Sp1 was used as a loading control.