Effect of overexpression of PU.1 on GATA-1 DNA-binding activity in K562 cells during erythroid differentiation.
Overexpression of PU.1 decreases GATA-1 DNA binding activity in K562 cells during erythroid differentiation. (A) Nuclear extracts were harvested from K562/PU cells at different time points after hemin induction (in the absence of ZnSO4 and PU.1 induction). As shown on the top panel, 1 μg of each preparation was used for EMSAs. The probe is the same as in Figure 3A. As shown in the bottom panel, 20 μg of each extract was used for Western blot analysis to detect GATA-1 protein expression. (B) Nuclear extracts were harvested from K562/PU cells at different time points after induction with both ZnSO4 and hemin. The amounts of protein used, probe, and antibody are identical to those described in Figure 4A. The bottom panel demonstrates the amount of GATA-1 protein expression.
