How PU.1 and GATA-1 inhibit each other's function.

PU.1 and GATA-1 inhibit each other's function through protein–protein interactions by different mechanisms. The top panel shows that GATA-1 inhibits PU.1 transactivation of PU.1 target genes (such as CD11b46) by blocking the binding of the PU.1 coactivator c-Jun to the β3/β4 region of PU.1. The bottom panel shows that the N-terminal region of PU.1 blocks GATA-1 function by binding to the GATA-1 C-finger and inhibiting GATA-1 binding to DNA.