Time course of the thrombin-induced release of AP from purified plasma FXIII of different Val34Leu genotype.

(A) The release of AP by 0.5 U/mL thrombin from wild-type (Val/Val, ■——■), heterozygous (Val/Leu, ┘ · · · · · · · ┘), and homozygous (Leu/Leu, ▪- - -▪) plasma FXIII. Total values represent the amounts released by 40 U/mL thrombin in 30 minutes at 37°C. (B) The release of 34Val (○——○) and 34Leu (●- - -●) AP from purified FXIII of a heterozygous control. (C) Elution profile of AP released from wild-type, heterozygous, and homozygous (upper, middle, and lower chromatogram, respectively) plasma FXIII by 0.5 U/mL thrombin in 20 minutes at 37°C. Retention times of wild-type and mutant AP are indicated. The elution profile was monitored at 210 nm.