Degradation of CTAP-III by activated neutrophil gelatinase B.

CTAP-III was incubated with activated gelatinase B in the presence or absence of the metalloproteinase inhibitors EDTA,o-phenantrolin (PHEN), and TIMP-1. The 3 inhibitors separately inhibited the degradation completely. Stromelysin-1 alone was not able to degrade CTAP-III. S indicates molecular mass standard. The symbols + and − in the upper line indicate the presence or absence of the indicated inhibitors, whereas the symbols 0, −, and + in the line underneath indicate no incubation, incubation with stromelysin-1 alone, and incubation with stromelysin-1–activated gelatinase B, respectively.