Time course of the degradation of IL-8 and CTAP-III by activated gelatinase B.

Recombinant IL-8 (A) and natural CTAP-III (B) (both at 4 μmol/L) were incubated with activated neutrophil gelatinase B (0.4 μmol/L) at 37°C. A control sample was incubated with activated stromelysin-1 (0.004 μmol/L). Samples were taken at the indicated time intervals and analyzed by SDS-PAGE and silver staining. S indicates relative molecular mass standard; the symbols − and + indicate incubation with stromelysin-1 alone and with activated gelatinase B, respectively.