Gelatinase B release and chemotactic activities of IL-8(1-77) and IL-8(7-77).

(A) Purified granulocytes were incubated with various concentrations of IL-8(1-77) and IL-8(7-77) for 30 minutes to induce the release of gelatinase B. Gelatinase B in the culture fluid of stimulated cells was analyzed by substrate zymography, quantified by scanning densitometry, and expressed in scanning units after the subtraction of background levels. Results are indicated as the mean (±SEM) of 3 independent experiments with neutrophil preparations from different donors. (B) The chemotactic activity of IL-8(1-77) and IL-8(7-77) for neutrophils was compared in a modified Boyden chemotaxis chamber. Chemotactic indices are shown as the mean and the standard errors of mean of 4 independent experiments with cell preparations from different donors.