![Fig. 11. Intracellular Ca++-mobilizing activity of IL-8(1-77) and IL-8(7-77) in CXCR1- and CXCR2-transfected cell lines. / Intracellular Ca++-mobilizing capacities of IL-8(1-77) and IL-8(7-77) were compared on cell lines transfected with CXCR1 and CXCR2. In both cell lines, IL-8(7-77) was more active than IL-8(1-77), but this difference was more pronounced in CXCR1- (A) than in CXCR2- (B) transfected cell lines (P < .05). One representative experiment of 3 is shown. Successive one-third dilutions of both IL-8 forms were analyzed until no increase in [Ca++]i could be measured. Detection limits in [Ca++]i increase were 50 and 20 nmol/L for CXCR1 and CXCR2 transfectants, respectively. For each IL-8 variant and CXCR interaction, dosages giving a response below the detection limit of the [Ca++]i increase were included.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/8/10.1182_blood.v96.8.2673/5/h82000242011.jpeg?Expires=1735684783&Signature=Y-hKp6X2cAzzj8e3Ishpci9PygF7C-QLgBXFSw5IN9VCXu4wny~RvKGjTUf8xC5yd7aTcevgRaoI-RBY59CAVGsqch3ec26laBLIPNG~kdb6jbhX8R8W4diitntwKn4xPrTF0xb933kZh3mMt4fb2rYfpEnokq2b7nNC~zhkyVNvuW1~xThjvpIp6WnEH17H~OGzoBdkHyXTDyoUG0vunHGQjSMvrF99O9l2v0OusfEmMwKqchP6g5-q4TEMc5OE0WlSKovqzk3ag5Mvzm1JoJro6r6ZishWRt8DhlwEGO8CO0bHZM217s~Qk2B3M5GBG5otXLOr0NSl~BjQV121UA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Intracellular Ca++-mobilizing activity of IL-8(1-77) and IL-8(7-77) in CXCR1- and CXCR2-transfected cell lines.
Intracellular Ca++-mobilizing capacities of IL-8(1-77) and IL-8(7-77) were compared on cell lines transfected with CXCR1 and CXCR2. In both cell lines, IL-8(7-77) was more active than IL-8(1-77), but this difference was more pronounced in CXCR1- (A) than in CXCR2- (B) transfected cell lines (P < .05). One representative experiment of 3 is shown. Successive one-third dilutions of both IL-8 forms were analyzed until no increase in [Ca++]i could be measured. Detection limits in [Ca++]i increase were 50 and 20 nmol/L for CXCR1 and CXCR2 transfectants, respectively. For each IL-8 variant and CXCR interaction, dosages giving a response below the detection limit of the [Ca++]i increase were included.
