GPI anchoring of DOK1.

(A) K562 cells were transfected with pDI2E construct, and stable transfectants were selected in G418-containing media. The cells were then incubated in PI-PLC (+) or buffer (−) prior to analysis. Flow cytometry was performed using FITC-conjugated anti-CD71, anti-CD59, and Dombrock antisera for comparison. (B) K562 cells and the GPI⁻ cell line transfected either with pI2E or pDI2E DNA and selected in G418-containing media were stained with Dombrock antisera and analyzed by flow cytometry. The mean fluorescence of each population is shown at the top of each panel.